Effectiveness of Using Brilliant Cresyl Blue Staining for Quality Evaluation and Developmental Competence of Immature and Mature Buffalo Oocytes

Al-shimaa Al-H. H. El-Naby, karima mahmoud, Youssef F. Ahmed, Mahmoud E.A. Abouel-Roos, Gamal A.M. Sosa

Abstract


The present study aimed to evaluate the effect of brilliant cresyl blue (BCB) stain on quality and developmental competence of mature and immature buffalo oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with 0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after maturation. The cleavage and blastocyst rates were determined. Results showed the percentage of BCB+: BCBˉ in immature and mature buffalo oocytes was 54:45 and 70:30 respectively. In immature oocytes percentage of embryo cleavage rate was highly significantly (P<0.0001) increased in BCB+ than BCB¯ oocytes. Lower cleavage rates were reported in immature BCB+ (P<0.05) and BCB¯(P<0.0001) compared to control non exposed oocytes. There was no significant difference between control and BCB+ oocytes in cleavage rate but there was a significant (P< 0.05) decrease in mature BCB¯ than control oocytes. In conclusion, BCB had a detrimental effect on immature buffalo oocytes, but had no effect on mature oocytes. Thus, it was recommended that BCB was not suitable for oocytes selection as it toxic, time and money consuming.


Keywords


Buffalo, oocytes, BCB, embryo development

Full Text:

PDF

References


Abazari-Kia, A. H., Mohammadi-Sangcheshmeh, A., Dehghani-Mohammadabadi, M., Jamshidi-Adegani, F., Veshkini, A., Zhandi, M., Cinar, M.U., & Salehi, M. (2014). Intracellular glutathione content, developmental competence & expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte. J. Assist. Reprod. Genet., 31, 313–321.

Alm, H., Torner, H., Lochrke, B., Viergutz, T., Ghoneim, I.M., & Kanitz, W. (2005). Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillantcresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity. Theriogenol., 63, 2194–2205.

Bhojwani, S., Alm, H., Torner, H., Kanitz, W., & Poehland, R. (2007). Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer. Theriogenol., 67, 341–345.

Bols, P.E.J., Jorssen, E.P.A., Goovaerts, I.G.F., Langbeen, A., & Leroy, J.L.M.R. (2010). High throughput non-invasive oocyte quality assessment: The search continues (Single oocyte culture & oocyte quality assessment). Anim. Reprod., 9(3), 420-425.

Brackett, B.G., & Oliphant, G. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. of Reprod., 12, 260- 274

Catala, M.G., Izquierdo, D., Uzbekova, S., Morato´, R., Roura, M., Romaguera, R., Papillier, P., & Paramio, M.T. (2011). Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity & embryo developmental competence in prepubertal sheep. Reprod., 142, 517–527.

Eppig, J., O’Brien, M., & Wigglesworth, K. (1996). Mammalian oocyte growth & development in vitro. Mol. Reprod. Dev., 44, 260–273.

Ericsson, S., Boice, M., Funahashi, H., & Day, B. (1993). Assessment of porcine oocytes using brilliant cresyl blue. Theriogenol., 39(1), 214.

Gasparrini, B. (2002). In vitro embryo production in buffalo species: State of the art. Theriogenol., 57, 237–256.

Heleil, B., & Fayed, M. (2010). Developmental competence of buffalo oocytes from follicles of different diameter selected by brilliant cresyl blue staining. Global Vet., 4(2), 176-184.

Ishizaki, C., Watanabe, H., Bhuiyan, M.M., & Fukui, Y. (2009). Developmental competence of porcine oocytes selected by brilliant cresyl blue & matured individually in a chemically defined culture medium. Theriogenol., 72,72–80.

Kempisty, B., Jackowska, M., Piotrowska, H., Antosik, P., Wozna, M., Bukowska, D., Brüssow, K.P., & Jaskowski, J.M. (2011). Zonapellucida glycoprotein 3 (pZP3) & integrin β2 (ITGB2) mRNA & protein expression in porcine oocytes after single & double exposure to brilliant cresyl blue test. Theriogenol., 75, 1525-1535.

Knobil, E., & Neill, J.D. (1988). The physiology of reproduction. Raven Press, New York, 1, 69-102.

Mahmoud, K.G.M. (2001). Cytogenetic studies on in-vitro fertilization in buffaloes. Ph. D. thesis.(Theriogenol.)Fac. of Vet. Med., Cairo Univ.

Mangia, F., & Epstein, C.J. (1975). Biochemical studies of growing mouse oocytes: Preparation of oocytes & analysis of glucose-6-phosphate dehydrogenase & lactate dehydrogenase activities. Dev. Biol., 45, 211–220.

Manjunatha, B.M., Gupta, P.S., Devaraj, M., Ravindra, J.P., & Nandi, S. (2007). Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM. Theriogenol., 68(9),1299– 1304.

Mohammadi-Sangcheshmeh, A., Soleimani, M., Deldar, H., Salehi, M., Soudi, S., Hashemi, S.M. , Schellander, K., & Hoelker, M. (2012). Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time. J. Assist. Reprod. Genet., 29, 153–158.

Nasr-Esfahani, M.H., & Johnson, M.H. (1992). How does transferrin overcome the in vitro block to development of the mouse pre-implantation embryo? J. Reprod. Fertil., 96: 41–48.

Niwa, K., & Ohgoda, O. (1988). Synergistic effect of caffeine & heparin on in-vitro fertilization of cattle oocytes matured in culture. Theriogenology, 30, 733-741.

Opiela, J., Katska-Ksiazkiewicz, L., Lipiński, D., Słomski, R., Bzowska, M., & Ryńska, B. (2008). Interactions among activity of glucose-6-phosphate dehydrogenase in immature oocytes, expression of apoptosis-related genes BC-2 & Bax, & developmental competence following IVP in cattle. Theriogenol., 69, 546–555.

Ozawa, M., Nagai, T., Somfai, T., Nakai, M., Maedomari, N., Miyazaki, H., Kaneko, H., Noguchi, J., & Kikuchi, K. (2010). Cumulus cell-enclosed oocytes acquire a capacity to synthesize GSH by FSH stimulation during in vitro maturation in pigs. J. of Cell. Physiol., 222(2), 294–301.

Pawlak, P., Warzych, E., Chabowska, A., & Lechniak, D. (2014). Differences in cytoplasmic maturation between the BCB+ & control porcine oocytes. Do not justify application of the BCB test for a standard IVM protocol. J. of Reprod., & Develop., 60(1), 28–36.

Pereira, G.R., Lorenzo, P.L., Carneiro, G.F., Bilodeau-Goeseels, S., Kastelic, J.P., Esteller-Vico, A., Lopez-Bejar, M., & Liu, I.K. (2014). Selection of developmentally competent immature equine oocytes with brilliant cresyl blue stain prior to in vitro maturation with equine growth hormone. Zygote., 22(4), 500-504.

Pujol, M., Lopez-Bejar, M., & Paramio, M.T. (2004). Developmental competence of heifer oocytes selected using the brilliant cresyl blue (BCB) test. Theriogenol., 61,735–744.

Rodrigues, B.A., Rodriguez P., Silva, A.E., Cavalcante, L.F., Feltrin, C., & Rodrigues, J.L. (2009). Preliminary study in immature canine oocytes stained with brilliant cresyl blue & obtained from bitches with low & high progesterone serum profiles. Reprod. In Domest. Anim., 44, 2255–2258.

Rodriguez-Gonzalez, E., Lopez-Bejar, M., Velilla, E., & Paramio, M.T. (2002). Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenol., 57, 1397–1409.

Ruvolo, G., Fattouh, R.R., Bosco, L., Brucculeri, A.M., & Cittadini, E. (2013). New molecular markers for the evaluation of gamete quality. J. Assist. Reprod. Genet., 30(2), 207–212

Salimi, M., Salehi, M., Farahani, R.M., Abadi, M.D.M., Novin, M.G., Nourozian, M., & Hosseini, A. (2014). The effect of melatonin on maturation, glutathione level & expression of HMGB1 gene in brilliant cresyl blue (BCB) stained immature oocyte. Cell J., 15(4), 294-301.

Salviano, M.B., Collares, F.J.F., Becker, B.S., Rodrigues, B.A., & Rodrigues, J.L. (2015). Bovine non-competent oocytes (BCB–) negatively impact the capacity of competent (BCB+) oocytes to undergo in vitro maturation, fertilization & embryonic development. Zygote., 1, 1-7.

Scholkamy, T.H., Darwish, S.F., & Mahmoud, K.Gh.M. (2015). Effect of verification by straw & cryotop on DNA integrity using comet assay with reference to brilliant cresyl blue exposure in buffalo oocytes. Alexandria J. of Vet. Sci., 46,117-123.

Shabankareh, H. K., Azimi, G., & Torki, M. (2014). Developmental competence of bovine oocytes selected based on follicle size & using the brilliant cresyl blue (BCB) test. Iran J. Reprod. Med., 12, 771-778.

Spikings, E.C., Alderson, J., & John, J.C. St. (2007). Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. Biol. Reprod., 76, 327–335.

Wang, L., Lin, J., Huang, J., Wang, J., Zhao, Y. & Chen, T. (2012). Selection of ovine oocytes by brilliant cresyl blue staining. J. of Biomed., & Biotechnol., 1,1- 7.

Wongsrikeao, P., Otoi, T., Yamasaki, H., Agung, B., Taniguchi, M., Naoi, H., Shimizu, R., & Nagai, T. (2006). Effects of single & double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos. Theriogenol., 66, 366–372.

Wu, Y.G., Liu, Y., Zhou, P., Lan, G.C., Han, D., Miao, D.Q., & Tan, J.H. (2007). Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model. Cell Res., 17, 722–731.

Zuelke, K.A., Jeffay, S.C., Zucker, R.M., & Perreault, S.D. (2003). Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes & pre-implantation stage embryos. Mol. Reprod., & Dev., 64,106-112.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Global Journal of Animal Scientific Research

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.